REQUEST FOR INFORMATION: SPRI Beads

With this request for information, HUS launches a market consultation to prepare a procurement. The aim of this prior information notice is to find suppliers operating in this field of business, get information of the solutions available in the market, and inform our plans and requirements for the forthcoming procurement. HUS Laboratory of Genetics at HUS (Helsinki University Hospital) Diagnostic Center is a SFS-EN ISO 15189:2022 accredited laboratory providing diagnostic and follow-up genetic testing services to all patients at Helsinki University Hospital area. The requested product is expected to be a DNA size selection reagent based on paramagnetic beads suitable for next generation sequencing (NGS) library preparation. It should utilize SPRI technology (Solid Phase Reversible Immobilization) for selective binding and purification of DNA fragments and enable the purification of sequencing libraries using magnetic separation. The estimated annual consumption of the reagent is approximately 2 litres. This estimate is provided for information purposes only and does not constitute a commitment to purchase any specific quantity. Intended Use: The intended use of the product is preparation and purification of NGS libraries compatible with Illumina and Ion Torrent sequencing instruments. Requirements: HUS is interested in products meeting the following requirements related to NGS workflows: 1. The reagent should enable efficient purification of NGS libraries within 150–1000 bp range. 2. The reagent should be compatible with the following NGS library preparation methods: - Ion AmpliSeq Library Kit 2.0 or newer - Ion AmpliSeq HD Library Kit - FusionPlex Reagents for Ion Torrent - Watchmaker DNA Library Prep with Fragmentation - Watchmaker RNA Library Prep Kit with Polaris Depletion 3. The magnetic separation should be efficient, allowing the separation of the beads and supernatant 2 minutes after placing onto the magnetic rack. 4. The reagent should be suitable for both manual and automated NGS workflows. 5. It should efficiently remove smaller DNA fragments, e.g. free adapters and adapter dimers, producing high-quality sequencing libraries as evaluated by fragment analysis and NGS quality metrics. 6. Library purification and yield should be highly repeatable across replicates and reagent lots. The Laboratory of Genetics will assess the performance of each lot. 7. The reagent’s performance should be in line with the SPRI beads currently in use in the laboratory. 8. HUS is interested in package sizes between 50 and 200 ml. 9. Respondents are requested to provide preliminary pricing information (EUR, VAT 0%) and indicative delivery times for the proposed products and/or solution. 10. Respondents are requested to provide the following information and documentation, if available: - Datasheet, Instructions for Use, Certificate of Analysis - Guidelines for optimizing size selection for specific NGS applications - Description of lot-to-lot quality control processes INSTRUCTIONS FOR ANSWERING We kindly request that respondents clearly address all requirements set out in sections 1–8, describe how each requirement is met, provide, to the extent possible, the information and documentation requested under sections 9 and 10, and identify and comment on any concerns, deviations, or proposed amendments to the attached draft agreement for the purchase of goods and its appendices. Respondents are also requested to provide the name and email address of a contact person for any subsequent one-to-one market dialogue and/or requests for additional information from the contracting authority. Please submit your response to this request for information by email to [email protected] no later than 7 September 2026. The subject line of the email must include: HUS 370-2026 SPRI Beads